Can I calculate the average from the data which are collected every 30 minutes for ANOVA?

I artifically elevated the air temperature in the heated plot by 3-celsius degree compared to that in the control for 7 days. I want to figure out the effect of the temperature manipulation on...

21 January 2021 1,185 8 View

What is wrong with my western blot transfer system? (smearing is occured)?

I'm now doing western blotting for several proteins. but transfer is not perfectly happened. bands are looks like smeared or smudged. And it seems to the lower MW bands are more affected. what did...

06 December 2020 9,153 5 View

How to calculate bulk density, porosity, and void ratio of soil?

If the particle diameter is 2 mm and the particle density is 2.5 g/cm, what is the bulk density, porosity, and void ratio of this soil? I had known that the formula to calculate them. The...

11 October 2020 3,466 3 View

How can I check the deparaffinization during IHC protocol?

Hello. I am a beginner researcher experimenting with IHC for the first time. I would like to know how to make sure it is completely deparaffinized when it is fixed with paraffin and removed with...

22 March 2020 7,795 3 View

If the cofactor(NAD) concentration in 1mM and substrate is 10mM in enzyme reaction, the substrate become 9mM after reaction?

If the cofactor(NAD) concentration in 1mM and substrate is 10mM in enzyme reaction, the substrate become 9mM after reaction?

02 March 2020 3,982 6 View

How to make simulated body fluid using glycerol and ethanol?

Hi. I'm trying to find the method making simulated body fluid using gylcerol and ethanol, but I cannot find it. (I want to make the solution that has similar surface tension and viscosity of human...

05 November 2019 9,051 1 View

How many cells do I need to get a pure plasma membrane? Do you have a kit?

The purpose of this study is to obtain plasma membranes from adherent cells by means of ultracentrifuge. The problem with the experiment is that the yield is too low when obtaining plasma...

01 October 2019 3,375 3 View

What is the difference between PET film and biaxially oriented PET film?

Hi. I'm trying to make LIS(Liquid Impregnated Surfaces) using PET film, so I was looking for the appropriate film product. Then I found biaxially oriented PET film, and I want to know if there is...

22 July 2019 329 1 View

Can I use multilevel regression with skewed count data?

I am studying neighbourhood effects on antisocial behaviour using multilevel modelling. The dependent variable, antisocial behaviour is a count data which varies between 0 and 10 which is a skewed...

28 November 2018 8,633 7 View

In sandwich ELISA in human serum, how can I reduce matrix effects and background?

Hello, I'm setting sandwich ELISA to measure amount of target protein in human serum. I have recombinant protein and checked 2 kinds of antibodes(different epitope) were suitable to perform...

29 March 2018 8,728 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View