Do you have any recommendations of a sofware or tool to align peptide fragments obtained by MS/MS?

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BD Cell Viability Kit - TO evaporation?

Hello, I'm conducting flow cytometry experiments using the BD Cell Viability Kit and I've noticed that the TO solution ends much faster than the PI solution. I store the solutions according to...

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Best method for extracting RNA from morulae?

Best method and number of embryos for extracting RNA from pig morulae?

21 July 2024 4,398 1 View

Para Antropólogos/as: ¿Quisiera ser entrevistado?

Hola, Soy una estudiante de primer año de la carrera de Antropología en Chile, siendo parte del alumnado de la Universidad de Concepción. Se me ha asignado junto con mi grupo el trabajo de...

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How to measure de expansion of the cumulus?

Hello, I would like to inquire about how the expansion of the cumulus can be measured during or after IVM. Thank you.

16 May 2024 9,330 2 View

The best media for IVP in mice?

Hello community! What is for you the best combination of media for IVM, IVF and CIV in mice? In my experience we usually use media made in our laboratory but I have the opportunity to purchase...

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BLAST sequence in plus/minus and plus/plus in reverse sequence when CRISPR is performed. Are they mutants?

We are performing CRISPR on a gene from a strain of Aspergillus niger and then sequencing the results by Sanger. When we compare the gene sequence with the resulting sequence we observe in the...

25 April 2024 6,159 0 View

Determination of Tocopherols and phytosterols by GC-MS without derivatization?

Hello, I need to determine tocopheroels and phytosterols by GC-MS but I do not have the reagents to derivatize (TMS, TMCS, BSTFA, etc.) that most of the consulted literature generally uses. I...

22 April 2024 8,178 6 View

Where can I get Biological Materials Science Book (Myers and Chen, 2014)?

I know it may be controversial, and I favour crediting the authors. However, knowledge should also be accessible to student researchers. My doctoral colleague and I would like to access the book,...

12 March 2024 1,333 4 View

How to calculate the sample size calculation for an in vitro acrylic resin study?

I need to make my sample size calculation without a pilot study, my n=10, but how can I prove that I have this number?

02 January 2024 9,534 1 View

How can I get calcium alginate at home with sodium alginate and calcium chloride?

Dear Researchers, I am looking for ratios of sodium alginate (powder) and calcium chloride (flakes/ granulate) and processes to create calcium alginate in FabLab facilities. PS: I am doing my...

12 December 2023 6,435 5 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

How to increase simulation box size?

We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...

08 August 2024 4,842 0 View

Unusual intensity drop in some sections of chromatograms in DDA?

Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...

07 August 2024 3,218 4 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

How can major oxides determined by ICP-MS/ES be normalized to 100% volatile free? How does this connect with LOI?

I am analyzing cherts from different sources found within one specific geologic formation using ICP-MS/ES. My committee was asking this question about my analysis. I was not sure how to answer this

05 August 2024 7,902 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

Usage of internal standards in LC-MS/MS analysis?

Have you ever seen a LC-MS/MS method uses both internal standards and external standards (in matrix matching purpose) but the concentrations of internal standards are outside the calibration curve...

05 August 2024 3,084 6 View

How to analyze multiple phosphorilation sites?

Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...

04 August 2024 8,432 3 View