I have to break yeast in order to collect the proteins for purification purpose. I have started 3L of cultures thinking that I had a protocol, but I don't have one...
I have a french press in which one I can put 40mL of sample at a time. So basically, I will pellet my culture, resuspend the pellet in my purification buffer and pass the sample in the machine.
My question is: is there a limit of density at which the lysis will be less effective? If so (an I guess there is), what it is?
If you have some advice, I will be glad to hear them and to discuss with you.