Do you have a detailed RT-PCR protocol?

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Saleh Alkarim

Hello

find the attachments & links here will help you

Good Luck

https://www.qiagen.com/sa/resources/molecular-biology-methods/pcr/

http://www.protocol-online.org/prot/Molecular_Biology/PCR/Real-Time_PCR/

Md Zulfekar Ali

I'm grateful to Saleh Alkarim and Laurence Stuart Hall for your kind descriptive and informative answer

Lin Zhang

I always use the protocol comes with the qPCR reagents.

Vikrant singh Chouhan

Md Zulfekar Ali,

I agree with the Lin you will go with the manufacture's instructions and also instrument you are using.

thanks to Chouhan

THANKS A LOT TO HALL