Do you coat petri dishes with 2% gelatin (Sigma G1393) when growing HUVEC-C (ATCC CRL-1730)?
Hi It is a longtime since I did any HUVEC work but as far as I remember a low gelling temp gelatin (and/or FN) is a requirement for primary cell isolation. For the cell lines some researchers do and don't coat see:
https://www.researchgate.net/post/Is_it_important_to_coat_the_flask_tissue_culture_plates_etc_with_ECM_like_gelatin_or_collagen_while_culturing_HUVEC
http://vrd.bwh.harvard.edu/core_facilities/cx_huvec.html
Hi,
We don't use HUVEC from ATCC.We isolate our own.We use 0.2% gelatin and this works best for us.
Hope this helps.
Hi Devrim,
we used to coat our flasks and dishes with gelatin when growing HUVEC, but we did it at 1% in PBS. We left it for 1h at room temperature, then removed the excess gelatin and washed once with PBS before seeding the cells. Hope that helps!
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