Do we have any cost effective in vitro method to screen anti-diabetic activity of small molecules?

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Francisco Javier Ortiz-López

You could try with this one

http://worldwide.espacenet.com/publicationDetails/biblio?CC=EP&NR=2600147A2&KC=A2&FT=D

Method for screening diabetes treating agent

EP 2600147 A2

It is supposed to be cost effective...

Cheers

Caroline Arous

You can easily take all cell lines from insulin-target tissue (adipocyte, muscle, hepatocyte) induced in vitro insulinoresistance by using palmitate or cytokines, and treat with your molecule. Insulinoresistance in tissue is caracterized by a decrease of insulin-induced phosho-Ser473 AKT. Then check whether your molecule rescue the cytokine/plamitate effect on phospho-AKT.