I am using stepone PLus machine and in excel sheet attached to my plate . i found effiency mentioned 1? what does this mean? iam quiet new to the device
Yes, relative quantification in qPCR is calculated using the delta-delta Ct method. The efficiency is how closely your primers match the desired rate of product formation. To publish your results, your primer efficiency must be 95-105%.
Here's a publication to get you familiar with the MIQE guidelines to publish your qPCR data:
Article The MIQE Guidelines: minimum Information for Publication of ...