In conventional cDNA preparation reaction , random hexamers or oligo (dTs) are added. However when I look at the protocols for isothermal viral genome amplification using RT-LAMP method, these oligos are nowhere mentioned. Also, is the cDNA preparation during RT-LAMP takes place simultaneously to the rapid amplification or does it happens in the beginning. Any help in understanding is appreciated.

Thanks.

More Ujjwal Ranjan Dahiya's questions See All
Similar questions and discussions