Phenotypic Microarray Mammalian (PMM) can be use to assess compound Mode of action on a specific cell lines. However we are not sure if we need to add the compound together with the dye or we add the compound, then only we add dye later?
Thanks
http://www.biolog.com/pdf/pmmlit/00P134rC%20PMM%20Broch%20PM-M1%20to%20-M14.pdf
http://www.biolog.com/pdf/pmmlit/Bochner_PLoS_ONE_2011.pdf
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Attached is the sample data
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