Do the targeting with ligand(s) for a particular receptor (s) always 100%?

11 February 2019 1 9K Report

When specific receptors are targeted for specificity, wouldn't there be still a fraction of the targeted stuff that are taken by the cells via non specific internalization?

Sabna Cheemadan

Thank you Richard!

Badges
Science method

More Sabna Cheemadan's questions See All

Tensile Speed and ultimate strength relation?

Hi all, How do you compare the tensile strength of a polymeric membranes tested at two different speed? I have a membrane tested at 20 mm/minute and the resultant strength is 3 MPa while a...

03 September 2022 6,785 0 View

Why are my western blot bands are think and faint?

Hi, my western blot bands are appearing at the correct molecular weight but are very thin and faint, making it difficult to distinguish difference in expression for shrna samples. Conditions...

04 January 2021 9,463 4 View

How to purify GABA from microbes?

I am working on neurotransmitter from microbes .

26 January 2018 763 1 View

Can anyone help with co-transfection of miR overexpression with its target?

In order to see the functional activity of my miR-over expressing constructs, I did a co-transfection of the over-expressing plasmid with its target reporter. I could hardly see much difference...

10 June 2014 5,810 5 View

Why should additional NaCl be added for nuclear protein extraction (nfkb isolation)?

I am trying to isolate a nuclear protein NF-kB from raw cells in order to do a western blot. I have searched some protocol for nuclear isolation and finally came up with one. But in that...

11 April 2014 2,261 3 View

What is the protocol for isolating NF-kB?

Hi, I am trying to isolate a nuclear protein, NF-kB, from raw cells in order to do a western blot. Can anyone suggest a protocol for isolating this nuclear protein? Thanks in advance, Sabna

25 March 2014 4,226 2 View

Why don't d-orbitals split themselves, why does it take a ligand? why don't protons from ligand repel nucleus split d-orbitals?

why don't d-orbitals split themselves because of themselves without the presence of ligands? Electrons are indistinguishable. Why wouldn't it be more correct that protons from a ligand split the...

03 August 2024 3,589 3 View

Why doesn't chromium 2+ ion use all its d-orbitals to receive lone pairs from six waters in [Cr(H20)6)]3+?

I'm guessing it's because the ligand experiences too much electron repulsion or proton repulsion from the chromium to insert them close to the 3d-orbitals which are close to the metal nucleus. Is...

03 August 2024 1,370 1 View

How can you draw a coordinate bond between a metal and donor atom in metal complexes, using discovery studio visulizer software?

Hello,, The metal complex ligand appears incomplete in the screen of the discovery studio visualizer

30 July 2024 3,354 1 View

AutoGrid and Autodock 4.2.6 window opens for 1 second then closes and there is no output files?

Good day, I am a student trying to work on Autodock for a project regarding Ligand-DNA interaction so i am quite new to molecular docking. i have followed tutorials and did all the steps...

28 July 2024 2,136 4 View

How to determine LOD values?

I am performing fluorescence experiments using a ligand to detect metal ions. I want to determine the Lowest Detection Limit (LOD) using the formula LOD = 3σ / K. However, I'm uncertain about...

19 July 2024 1,086 1 View

Not able to give GAFF forcefield to my ligand?

I have been able to assign the CHARMM forcefield to my ligand i.e. acetyl coenzyme A but not the amber forcefield. I want to run amber forcefield and the parameterization of ligand is failing. I...

15 July 2024 7,323 1 View

Printing residues in an object in Pymol?

Hi! I am currently working on a protein where I have selected residues within 10A of its bound ligand in pymol. However, I can not find a way to print out what residues are contained in that group...

15 July 2024 1,727 1 View

How can we identify (in silico) the interacting amino acid residues or the nucleotides involved in the Protein-Protein / Protein-RNA interaction?

Hello! everyone, I am trying to study in silico Protein-Protein and Protein-RNA interactions. Now, is there any tool with which I can identify the interacting amino acid residues or the...

14 July 2024 950 2 View

Does HEK293T cell express RIG1?

Is there any benefit in knocking out the interferon receptor in 293T cells? In the context of creating RNA viruses 📷

13 July 2024 3,382 0 View

Why Cl atom is not showing in docked comlex of molecule?

During the preparation of a ligand molecule in autodock or openbabel Cl atom is not showing in the .pdbqt file whereas it was present in the pdb and sdf file. Due to this Cl atom is not showing in...

13 July 2024 3,486 6 View