27 January 2018 1 713 Report

I am working on neurotransmitter from microbes .

More Sabna Bs's questions See All
Why are my western blot bands are think and faint?

Hi, my western blot bands are appearing at the correct molecular weight but are very thin and faint, making it difficult to distinguish difference in expression for shrna samples. Conditions...

04 January 2021 9,204 4 View

Is there any interconection and similarities of Innate Intelligence, Innovativeness and Creativiity ?

There is a scholarly controversy/ different opinions as to whether Intelligence is a product of hereditary or environment of human being.

14 November 2018 8,396 2 View

Can anyone help with co-transfection of miR overexpression with its target?

In order to see the functional activity of my miR-over expressing constructs, I did a co-transfection of the over-expressing plasmid with its target reporter. I could hardly see much difference...

10 June 2014 5,725 5 View

Why should additional NaCl be added for nuclear protein extraction (nfkb isolation)?

I am trying to isolate a nuclear protein NF-kB from raw cells in order to do a western blot. I have searched some protocol for nuclear isolation and finally came up with one. But in that...

11 April 2014 2,153 3 View

What is the protocol for isolating NF-kB?

Hi, I am trying to isolate a nuclear protein, NF-kB, from raw cells in order to do a western blot. Can anyone suggest a protocol for isolating this nuclear protein? Thanks in advance, Sabna

25 March 2014 4,138 2 View

Dynamically allocating the resource in cloud environment?

What are the efficient algorithms needed for the comparison and how do we implement?

10 March 2014 9,284 2 View

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