How to purify GABA from microbes?

27 January 2018 1 700 Report

I am working on neurotransmitter from microbes .

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More Sabna Bs's questions See All

Why are my western blot bands are think and faint?

Hi, my western blot bands are appearing at the correct molecular weight but are very thin and faint, making it difficult to distinguish difference in expression for shrna samples. Conditions...

04 January 2021 9,204 4 View

Is there any interconection and similarities of Innate Intelligence, Innovativeness and Creativiity ?

There is a scholarly controversy/ different opinions as to whether Intelligence is a product of hereditary or environment of human being.

14 November 2018 8,396 2 View

Can anyone help with co-transfection of miR overexpression with its target?

In order to see the functional activity of my miR-over expressing constructs, I did a co-transfection of the over-expressing plasmid with its target reporter. I could hardly see much difference...

10 June 2014 5,725 5 View

Why should additional NaCl be added for nuclear protein extraction (nfkb isolation)?

I am trying to isolate a nuclear protein NF-kB from raw cells in order to do a western blot. I have searched some protocol for nuclear isolation and finally came up with one. But in that...

11 April 2014 2,153 3 View

What is the protocol for isolating NF-kB?

Hi, I am trying to isolate a nuclear protein, NF-kB, from raw cells in order to do a western blot. Can anyone suggest a protocol for isolating this nuclear protein? Thanks in advance, Sabna

25 March 2014 4,138 2 View

Dynamically allocating the resource in cloud environment?

What are the efficient algorithms needed for the comparison and how do we implement?

10 March 2014 9,284 2 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

How to indicate the degree of litter decomposed by microbes?

Any technique could detect the degree of litter decomposed by microbes? Or any indexes could do this job? I want to split the contribution of microbes during decomposition, so any suggestions...

23 February 2021 1,362 5 View

Can I get information about research laboratories that are currently having in culture, internationally/locally standardised microorganisms?

Microorganisms of interest include; E.coli; Salmonella typhimurium; Pseudomonas aeroginosa; Streptoccocus pneumoniae; Staphyloccocus aureus and Candida albicans. Locations closer to Port...

18 February 2021 6,500 3 View

How do microbes tell the presence of an inorganic minaral in the soil?

Due to their ubiquitous nature, there is a tendency of the presence of microbes in a given mineral(s). For instance, if microorganisms that love the of pH less than 6 in the pH value, is isolated...

03 February 2021 4,630 10 View

How to lessen cell growth?

I am working on the 4T1 murine breast cancer cell line. from the first day I bought this cell line, the cell's growth rate is extremely high. I intend to make these cells resistant to a single...

24 January 2021 2,884 3 View

I am looking to develop a basic oral microbiome assay. Can anyone advise on culture vessel, conditions and organisms that require minimal maintenance?

I am looking to investigate oral care products with an easy-to-prepare culture of orally derrived microorganisms.

21 January 2021 3,448 1 View

How can I prepare 300mg/L concentration of lead(II) nitrate ( Mol. Wt reffered is 331.21) incoorporated Luria-Bertani (LB) agar ?

I need to check the Heavy metal (lead) consumption assay using microbes at varying concentration in same media. ( 200mg/L to 2000mg/L).kindly help me to clear it.

19 January 2021 1,946 9 View

Why below-ground ecology is still unexplored (majority of it) in our country ?

Below ground ecology or below ground ecological functions and ecosystems roles are very crucial and highly significant for any ecosystem especially for the forest ecosystems. The interaction among...

02 January 2021 6,912 3 View

What are the role of microbes for green revolution or modern advancement in science ?

We all know the role of microbes its miniature of the miracle how it can be , used in revolutionizing science in various fields.

23 December 2020 6,220 10 View

For a new biofertilizer what efficient methods should I follow to test it's quality(non-presence of pathogens, types &number of symbiotic microbes)?

Also it's effects on plants.

22 December 2020 2,318 3 View