If a mutant cell is cultured in a medium for several batches then what happens in case of the physiology and protein expression level?
If you are referring to the passaging of the cells, it is known to impact a lot of factors from transfection to response to drugs.
https://www.atcc.org/~/media/PDFs/Technical%20Bulletins/tb07.ashx
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2001501/
In deed, many factors will be affected regardless of the cell line, type of culturing, growth adaptations, particular medium, etc. Effects such as lowered cell viability, decreased expression, altered glycosylation pattern, aggregation in the media, incomplete signal peptide processing, protein clipping, and so many other effects. Each product and process will be different.
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