M. Manirujjaman

5 Questions 15 Answers 0 Followers

Questions related from M. Manirujjaman

How bacterial genome can be replaced with genetically modified plasmid?

After transformation how the bacterial own genome can be removed completely from the viable cell? It would be highly appreciated if someone provide information regarding this query.

08 August 2019 1,529 13 View

Hi every one,How long I can preserve PDCD4 mutant plasmid at 4 degree Celsius temperature?

I usually store plasmids at -20 degree Celsius

10 October 2016 3,794 0 View

Is there any method or reagent to isolate proteosome or phagosome?

Dear all,I want to isolate intact and active proteosome and phagosome,would you please help me in this regards?

06 June 2016 7,154 0 View

How long it takes for complete polymerization of SDS-PAGE gel?

How long it takes for complete polymerization of SDS-PAGE resolving (separating) gel? After pouring into the plate it takes one hour for polymerization,is it enough for experiment?

05 May 2016 9,507 20 View

Do the physiology and protein expression level of cancer cells in culture medium change with the number of batches?

If a mutant cell is cultured in a medium for several batches then what happens in case of the physiology and protein expression level?

03 March 2016 5,252 2 View