Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Please. Could you help be in implementing Fractal Discrete Cosine Transform algorithm in Matlab?

I do need the Matlab code of Fractal Discrete Cosine Transform (FDCT). Can anyone who has already implement this code, help me with the implementation of this transform?

24 February 2021 5,602 2 View

Monte Carlo simulation?

Hi, i am conducting a Monte Carlo simulation for my new distribution but the MSE values are increasing while the sample size increase, does any experience this issue before and what should i do...

22 February 2021 3,680 4 View

What is the reason film thickness increased?

what is the reason film thickness increased when increased doping concentrations give some reasons The measured film thickness is 156, 282, 394, 548 and 681 nm for 0, 1.5, 2.5, 3.5, and 4.5 wt....

17 February 2021 8,987 5 View

Is There are any idea to achieve H NMR signals for swollen compound ??

the samples are not completely soluble - just swelled the solvents that's mean the sample may be partially soluble any suggestion, please

16 February 2021 8,801 3 View

Are there any papers that deal with the effects of climate change on the pollination in Tropical orchids?

There are a few papers dealing with the effects of climate change on the pollination of European orchids, but I wonder if similar studies have been carried out in the Tropics.

04 February 2021 1,818 4 View

How to report path analysis / SEM with Yuan-Bentler correction from R output?

Dear all, I am currently writing my master thesis where I am analysizing my data using path analysis and SEM in R (lavaan package). Now that I like to write up my results, I am struggling with...

04 February 2021 8,993 2 View

Queries regarding the rain flow counting algorithm?

02 February 2021 9,790 2 View

Is there a way to get rid of TEA in a TLC plate in order to do proper staining?

Dear colleagues: I have been trying to establish a few HP-TLC methods in the lab. In general, for quantification purposes, I prefer CuSO4+acid charring, to primuline staining. Lately I have been...

20 January 2021 9,019 1 View

What is corrct way to define ports when simulating FSS and surface waves in CST?

I need to simulate a mushroom FSS and I'm struggling with the ports. What's the correct way to define them? Should I construct a proper SMA connectors, or is it enough to put a waveguide ports...

19 January 2021 6,590 4 View

Is it possible to have two luminescence bands of the same peak position but with different intensities in single spectrum??

Hi! If it is possible to have two luminescence bands of the same peak position in a spectrum, what is the reason behind formation of two separate bands instead of one? This phenomenon is related...

26 February 2021 4,963 5 View

What are the merits and demerits of GAN and classical data augmentation in plant leaf disease detection and classification systems?

anyone suggest what are the merits and demerits of GAN and classical data augmentation in plant leaf disease detection and classification systems

21 January 2021 5,135 5 View

GAN as Data Augmentation?

Hi, I want to apply GAN as a data-augmentation technique in my model. I am bit confused regarding implementation that how could I give the generated images of GAN to my model on run-time? because...

07 December 2020 4,703 3 View

Why use STOP solution in ELISA?

At the last step for data analysis, we always use a STOP solution to stop the TMB oxidized by HRP. Why do we have to stop the reaction? Couldn't we measure the color wavelength of...

17 November 2020 5,177 3 View

What would cause Cell Titre Glo assay wells to luminesce less than the media only wells?

Hi all, I'm doing a bunch of Cell Titre Glo assays to assess the effects of several inhibitors on 2 cell lines. Problem is, for some of the wells they seem to be luminescing less than the...

30 October 2020 1,513 2 View

Can anyone tell me that how to find dangling bond in GaN, AlN or AlGaN?

I want to know about the dangling bond? by which method we can find it, does its need some special type of characterization just like XRD etc... or method i want to find dangling bond effect in...

26 October 2020 1,938 6 View

How I can find the quantum efffcincy from the measured and calculated decay time when the measured decay time is greater than the calcualted decay ?

I am working on the luminescence properties of Nd3+ ions doped phosphate glasses for which I have to study the decay time. I measured the decay time experimentally and also calculated the decay...

16 October 2020 951 7 View

How to find molecular energy transition from given chemiluminescent emission wavelength?

Hello, As part of my master's thesis, we are gasifying biomass in an entrained flow reactor. We are collecting the light emitted by the flame via a collimating lens and an optical fibre in order...

09 September 2020 1,262 2 View

I have got the following photo luminescence spectra data of AU-PD tio2 film for diffrent depotion rates ,can some one help me to analyse it broadly?

i have got the following photo luminescence spectra data of AU-PD tio2 film for diffrent depotion rates ,can some one help me to analyse it broadly? can we discuss it ??

02 September 2020 3,260 6 View

How to avoid getting organic particles to charr(become black) during calcination?

I am trying to synthesize SrHfO3:Eu2+ nanoparticles. To reduce from Eu3+ to Eu2+, we calcinate it under Ar/H2 conditions but it ends giving a black coloured product. We need this for luminescence...

22 August 2020 448 3 View