Do the AMV or M-MLV-RT (or derivatives) have any ligation or strand displacement activities?

22 October 2013 1 7K Report

Namely, what happens if a processing of RT enzyme meets the 5' end of a downstream primer (e.g. during a random hexamer RT)? Will the RT enzyme link the 5’ end of a hexamer to the growing cDNA strand?

Krish Kizhatil

As far as I can remember AMV displaces about 50 nt. not too sure about hexamer think it would depend in position on template.

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