We want to patch clamp cells with GABA A receptors, without the need to transfect the cells first. A colleague told me that N2A cells express GABA receptors without the need for transfection, but I'm having trouble finding papers that confirm this. Do the cells need to be differentiated to neurons before they'll express GABA A receptors, or do they already express these as primary cultures?
Yes, they do express GABA A receptors. For example, the following paper examines the expression of GABAA receptor γ2 subunit in N2A cells:
http://www.ncbi.nlm.nih.gov/pubmed/22119642
I remember they have GABA receptors, but not have miniature activity
Cheers
Dear Professor, We studied also early neural development in the avian embryo. We discovered that early neural development (pre-neurulation) is provoked by induction by the endophyll (primary hypoblast).Indeed endophyll orients and organizes the early head region (Callebaut et al.,1999: Eur. J. Morphol. 37 : 37-52. On the other hand by placing a quail Hensen's node (Stage 4-5 V) on the deep side of the upper layer of an isolated anti-sickle region from unincubated chicken blastoderms, we observed the appearance of a quail floor plate, a quail chorda and also quaill cells in the median roof of the definitive endoderm. ( Early steps in neural development : Callebaut et al.,2006: J. Morphol. 265 : 793-802. See our E-book : From ooplasms to embryo : The "embryo in box" model (Edit. acco, Leuven, Belgium).With kind regards.
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