Data Analysis K-S and S-W tests?

Hello, I have conducted analysis where the K-W and S-W have come out as p

25 February 2021 9,648 3 View

Simple clearing method for observation of hand-sectioned wheat leaves?

I am using a toluidine blue O preparation for staining different parts of wheat plants to observe a fungus growing in them. This works really well for lighter tissues of the plant! However, the...

27 October 2020 9,159 1 View

How to Perform Feature Selection with Multilevel Encoded Categorical Variables?

I'm attempting to do feature selection on the variables on my dataset, but unfortunately the overwhelming majority of them are categorical. Currently they've all been encoded into binary...

29 July 2020 7,581 3 View

3D Slicer to ANSYS or FEBio?

Hi all, Does anyone have a workflow for preparing volumetric meshes in 3D Slicer and importing them into finite element software such as ANSYS or FEBio? Been trying so many approaches but they...

26 July 2020 7,012 3 View

How do I report Multiple Regression Analysis with imputed values SPSS?

I'm conducting multiple regression in SPSS with data where I've replaced missing values using Multiple Imputation. The output gives the pooled values in the coefficients table, but for the model...

10 June 2020 2,069 2 View

Is there a way to set random initial values for occupancy models in R using the RPresence package? Or any documentation file for RPresence?

I am trying to set random initial values for some occupancy models in R, in response to getting some convergence warnings. I am using the RPresence package but cannot find any documentation files...

09 February 2020 3,057 2 View

Removing vitreous from baby rat eyes?

I have been trying to remove the vitreous humour from baby rat eyes (post natal day 15) and have been still having a lot of interference in my IHC staining. Does anyone have a good protocol to...

20 January 2020 4,634 1 View

Colony Formation assay vs Growth assay - do they report different things?

I'm looking at the response of an established cell line to addition of a drug in terms of growth inhibition. Whilst I see a slight growth inhibition when seeding cells 10-25% confluent (I have...

08 December 2019 319 1 View

GDNA stability at 55 degrees?

I was extracting genomic DNA from Streptomyces the previous day and left my gDNA in the oven (55 degrees Celsius) to dry at the very last step. I had to rush off for a seminar and completely...

11 September 2019 9,441 2 View

How does freezing antibiotics result in loss of activity?

I remember my mentor saying before that she decided to refrigerate her gentamycin stock solution instead of freezing it since that was the recommended storage condition. Now, I'm dealing with...

04 August 2019 5,766 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

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22 February 2021 9,932 11 View