Has anyone serviced their own micromanipulators? We have several Narishige MM-33 style manipulators that are drifting or are very difficult to move. I'd love to tighten and oil them myself to save money. Anyone have suggestions?
We are looking to inject a single dose into PND21 rats stereotactically via the ICV space. I know rats at PND21 are a bit larger than adult mice, so what is the maximum feasible amount of...
01 February 2020 3,154 2 View
I'm curious if anyone has sent mice to a company for EEG testing? We don't have the room to buy all new EEG equipment nor do we need to buy it since it is for a pilot study.
31 December 2019 1,511 3 View
Is that a long-shot? We have a CRISPR-Cas9 deletion rat that I find sketchy with our phenotyping. According to the company we got them from, they sequenced a region that shows a ~90 base pair...
04 May 2019 7,850 1 View
I've been studying field hippocampal electrophysiology in mice for many years and we've recently started working in rats. I am beyond frustrated because things do not work at smoothly in rats and...
01 February 2019 397 9 View
Hey Everyone, I have a CRISPR-Cas9 rat model that we genotype to verify heterozygotes. The company that designed the rats gave us the primer sequences (2 for genomic and 2 for knockout) and we...
09 October 2018 744 0 View
We recently started working in a rat model and perform decapitations as well as perfusions for IHC. We normally do our work with mice and therefore our scissors are designed for smaller animals....
05 June 2018 3,812 4 View
Hi Everyone! I'm studying a new genetic deletion rat model (Sprague-Dawley background) and we are looking to test them in Fear Conditioning. I'm used to working with mice so this is a bit new for...
01 February 2018 1,385 1 View
I want to do wettability analysis of hydrocarbon oil (octane)/rock/surfactant solution system. but to do the analysis first I have to make rock surface oil-wet. Since hydrocarbon oil does not have...
27 February 2021 5,383 3 View
What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?
24 February 2021 8,219 2 View
Oil and gas companies run gamma ray tool and take readings in API units while Gamma ray is also noted in cps, so how these units can be converted into each other?
24 February 2021 7,545 2 View
How can I remove my compound from paraffin oil? Mistakenly my compound fell in an oil. Compound = Europium complex
22 February 2021 5,667 2 View
During my trial for lubricant oil re-refinery process, I use NaOH as coagulant agent. After overnight of gravity settling, a solid layer in the surface was form. what product is that? since the...
22 February 2021 2,116 4 View
I have a mixture of epoxidized oil and magnesium sulfate wich type of filtre paper should i use
21 February 2021 3,944 3 View
Hello, I am investigating properties of water in oil emulsion explosives. And adding hexamine (hexamethylenetetramine) into one of the phases that are used to make the emulsion should increase the...
21 February 2021 8,680 1 View
I am studing the mircogel at oil-water interface. I want to know the adsorb amount of microgel at the oil water interface. How we can determine this amount?. I will highly appreciate your...
19 February 2021 2,787 1 View
I would like to quantify the lesions on aorta. I want to take image of atherosclerotic plaques formation in aorta by using O red Oil Solution. After the staining, what will be the storage...
16 February 2021 5,876 2 View
i am purifying cycle sequencing PCR reaction. i have tried multiple methods given in BDT manual (provided with the instrument) .everytime i used 96 well reaction plate for this purpose. 7 wells...
14 February 2021 8,534 2 View