8 Questions 10 Answers 0 Followers
Questions related from Melinda Peters
We are looking to inject a single dose into PND21 rats stereotactically via the ICV space. I know rats at PND21 are a bit larger than adult mice, so what is the maximum feasible amount of...
02 February 2020 3,232 2 View
I'm curious if anyone has sent mice to a company for EEG testing? We don't have the room to buy all new EEG equipment nor do we need to buy it since it is for a pilot study.
01 January 2020 1,566 3 View
Is that a long-shot? We have a CRISPR-Cas9 deletion rat that I find sketchy with our phenotyping. According to the company we got them from, they sequenced a region that shows a ~90 base pair...
05 May 2019 7,910 1 View
I've been studying field hippocampal electrophysiology in mice for many years and we've recently started working in rats. I am beyond frustrated because things do not work at smoothly in rats and...
02 February 2019 465 9 View
Hey Everyone, I have a CRISPR-Cas9 rat model that we genotype to verify heterozygotes. The company that designed the rats gave us the primer sequences (2 for genomic and 2 for knockout) and we...
10 October 2018 804 0 View
We recently started working in a rat model and perform decapitations as well as perfusions for IHC. We normally do our work with mice and therefore our scissors are designed for smaller animals....
06 June 2018 3,871 4 View
Has anyone serviced their own micromanipulators? We have several Narishige MM-33 style manipulators that are drifting or are very difficult to move. I'd love to tighten and oil them myself to...
05 May 2018 3,305 0 View
Hi Everyone! I'm studying a new genetic deletion rat model (Sprague-Dawley background) and we are looking to test them in Fear Conditioning. I'm used to working with mice so this is a bit new for...
02 February 2018 1,452 1 View
