Chamath Chandrasekera

3 Questions 1 Answers 0 Followers

Questions related from Chamath Chandrasekera

How to do PCR following RNA isolation and reverse transcription?

I need to compare gene expression of a gene in two differently treated samples. I isolated total mRNA using a QIAGEN column kit. 1. Do I use nanodrop to measure isolated RNA concentration and do...

06 February 2018 6,358 2 View

Why do SW620 cells grow very slowly.

I am growing SW620 cells in L-15 media supplemented with 10% FBS and pen strep. However 1ml of a fully confluent plate subcultured takes over a week to become fully confluent. Any reason why this...

16 June 2017 750 2 View

Do I use ethanol acetone to fix and permeabilize cells for transwell migration assays

I am wondering if I should fix and permeabilize my cells with a 50:50 mixture of ice cold methanol and acetone when I perform transwell migration assays. I have seen most methods mention using...

14 June 2017 8,353 2 View