So I have target cells to transduce gene for a VSV-SARS-CoV2-GFP spike pseudotype and have lower infections/transduction of genes when removing the inoculum, however the infection is more synchronized (more cells with similar GFP expression). I'm able to concentrate the virus and get around 20% GFP positive while I get around 8% when washing away. I see mixed methods in papers whether to wash or test entire inoculum or just not give details in the methods. I will be testing neutralization of infection and changes in protein expression after 24hr transduction by IF, flow and WB.

Thank you for your time,

Dale