RNA sequencing and plasmid copy number?

Hi everyone, I am in the very initial stages of planning an RNA sequencing experiment. I want to find out the differential gene expression in my bacterial strain of interest when exposed to...

12 June 2020 5,966 3 View

What method do I need to use if I would like to see whether different forms of the same protein (mutant, WT) bind more or less to another protein?

I have two proteins of interest - one is GFP-tagged and one is mCherry tagged. Each protein has several different forms (wild-type, mutant). My hypothesis is that the wild-type (not the mutant)...

19 February 2019 5,889 3 View

Do fixed Orthodontics reduce the oral pH?

08 February 2018 5,340 3 View

What is the best concentration and duration of treatment with anti-NKG2D(CX5) antibody in mice?

20 March 2017 8,448 3 View

Could anyone suggest why I get no colonies on control or sample plates after site directed mutagenesis?

10 February 2016 5,087 3 View

Are you familiar with the history of the food diary? Is there any specific research or works in literature and art on the food diary or journal?

28 October 2014 7,801 15 View

What's the best activation marker antibodies to use for pig platelets?

I'm planning to investigate the activation status of porcine (pig) platelets as a response to various stimuli. What antibodies would you recommend for flow cytometry?

12 February 2014 9,463 5 View

Can someone recommend a multicolour immune cell activation flow cytometry panel?

I'm looking to monitor immune cell activation (neutrophils, monocytes, T/B/NK-cells) using multicolour flow cytometry and was wondering if anyone can recommend already established panels?

07 February 2014 3,999 10 View

Can you recommend any courses or books about Japanese calligraphy?

Cynscribe Directory http://www.cynscribe.com/ is the most comprehensive online resource for calligraphy. However, many of the links in the section online videos/courses are broken and it is not...

04 January 2014 7,165 4 View

Funding and resources for artists, culture professionals and journalists

A recent post by Science Writers directed my attention to some very interesting guides and resources in the field of journalism as well as art/cultural mobility, that is mobility of artists,...

29 December 2013 6,405 2 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

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02 March 2021 3,060 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View