My question is if it is possible to analyse ELISA results similarly to what we do by WB. I have two ELISA kits for 2 different proteins. One of them is the internal control, IC (like actin or tubulin in a WB) and the other is my protein of interest, PI, susceptible to changes. The same sample will be run then using the 2 kits in paralel. I am interested in the ratio between the absorbances for both proteins in the same sample, and then compare the control-sample values to the different experimental conditions. I understand that the quantitave absolute value can not be obtained unless you use a standard curve, however I want to know the relative abundance of my PI/IC among the different conditions to be analysed. Most of publications just show changes in the total amount of a single protein assuming equal loading for all conditions. This is not a suitable case for us and we need to relativize to a loading control. Will absolute values after a STD curve give me any extra info than the ratio of absorbances? Can I just not measure relative absorbances and give 1 to control and compare to the other experimental conditions? wouldn't the "proportionallity" of changes in total amount of protein or the ratio of absorbance be the same?

Thanks!