Dear all,

I need to isolate nuclei to count them with a flow cytometer. I want to use a fast protocol using PBS and 0.1% NP-40 to pellet the nuclei. To resuspend the pellets before staining with PI (propidium ionide) in most protocols that I read they use the following recipe : 0.25M sucrose, 1mM CaCL2, 10mM Tris-Hcl and 0.1% NP-40.

I don't really understand why at this step they use NP-40 ?

Does  0.1% NP-40 lysis nuclear membranes ?

Thank you,

Cheers.