Do I have to use 0.1% NP-40 to resuspend nuclei before nuclear staining ?

Dear all, I need to isolate nuclei to count them with a flow cytometer. I want to use a fast protocol using PBS and 0.1% NP-40 to pellet the nuclei. To resuspend the pellets before staining with...

17 March 2017 4,241 1 View