Dear Emel,

I would always spin down tubes containing liquid before opening them to bring all sample to the bottom. Regarding vortexing, I would be very cautious with any protein sample. In case you want to make sure the sample is well mixed, I would just snip it and then spin down, or first spin and then carefully pipet up and down without forming air bubbles.

I hope this helps.

Hi @Emel,

I agree with @Heide Marie Resch with regard to avoid vortexing the protein samples like antibodies or enzymes. Vortexing can affect the quality of proteins and denature it. Short spins can be performed, just to bring all the sample from walls to bottom. For mixing, you can perform a gentle pippete in and out.

Good luck

Hi Emel,

May be gentle vortex doesn't do any harm to their nature. You need to be careful about it or just do a quick spin at the time of use.

But if you persistent for longer vortex sessions it may cause changes to the nature of antibody.

Best,

Hamid