Emel Karakaya

4 Questions 3 Answers 0 Followers

Questions related from Emel Karakaya

Do I have to centrifuge before using fluorophore conjugated antibodies? Will vortexing fluorophore conjugated antibodies harm them?

07 July 2020 7,344 3 View

Should FMO controls be prepared for all samples to be used in the experiment or is it sufficient to prepare for a single sample?

In the flow cytometry experiment I will perform soon, I will use the unstained control to detect autofluorescence, the single stain control as a compensation control to determine the degree of...

23 June 2020 5,408 4 View

When doing antibody titration for flow cytometry, is the unstained control sufficient as a negative control, or isotype control also required?

31 May 2020 5,176 7 View

Can I use two different antibodies conjugated with the same fluorophore in flow cytometry analysis?

I am trying to do flow cytometric analysis of exosomes for my thesis work. The flow cytometry device I use can only distinguish 3 colors. I think of using each color for a different purpose. I...

29 April 2020 2,039 3 View