We are planning on doing a contractility assay using H9c2 tomorrow for the first time. We are basing our experiments on the article by Ku et al ( Cardiovascular Diabetology 2011, 10:57). I have attached the article where a contraction assay for H9c2 cells is described. I hope this helps.
Have you successfully induced H9C2 to beat? The paper by Ku et al. seems to look at contractility in long time scale and may not represent the beating part faithfully. Just wondering if you have successfully induced the beating.