Though there are several animal studies but detailed method of anesthesia was not mentioned. So I need a method with proper reference.
Ketamine‐Xylazine K: 75‐150 mg/kg + X: 16‐20 mg/kg IP or SQ (in same syringe)
Dear Dharmendra Arya
You can use Halothane too. Just put some Halothane in a 50ml tube with some tissue to absorb easily and place the head part inside the tube.
https://www.researchservices.umn.edu/services-name/research-animal-resources/research-support/guidelines/anesthesia-rats
you can find the answer
to measure the effect of thermal burns , we had to go to the ethic commitee in France and they decided upon 2 protocole or anesthesia
This was 20 years ago I think they used ketamine..I must find the docuement but i am traveling a lot so if interested , please re contact me
Jean DB
Interesting answers…Opinions and philosophy are the last things that are needed here. I am really wondering who is doing "your" experiments? Some slave-students?
Dharmendra,
1. Any extreme overdose of an anesthetic given i.p. (intra peritoneal – which is easy for the beguilers) will do.
2. You may accelerate/induce the exitus when animal is deep in anesthesia, by quick laparatomy and section of aorta.
3. If the animal is in deep anesthesia already, intracardial injection of saturated KCL about 0.3ml or more is the best.
Look up and cite our papers on microcirculation or in vitro contractility.
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