Hotis Test for Mastitis
Hotis Test is supposed to be of a useful disgnostic procedure.
0.5cc of a sterile 0.5% aqueous solution of brom-cresol purple is added to 9.5cc of milk previously measured into a sterile test tube. The tube is inverted several times then incubated 24 hours at 370C.
When the dye and milk are first mixed, the color is purple. If streptococci are present the color changes during incubation from purple to green or yellow as a result of acid production. If the Strept. agalactiae is present small flakes or balls of growth canary yellow in color became deposited on the side of the tube.
The above may prove to be a useful test but must like other tests, supplement clinical examination. When possible the clinician should make a diagnosis with the assistance of laboratory tests but never should a laboratory test for mastitis be expected to replace the clinician. It can only be of
assistance-particularly a negative finding-when taken into consideration with the results of physical examination.
https://archive.org/details/hotistestfordete672mill
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