Why does flow deformation occur at the test line and control line on nitrocellulose membranes (LFIA)?

I use 1% BSA in 10 mM phosphate buffer (pH 7.4) solution when striping my nitrocellulose membranes with antigens and antibodies. However, I always observe deformed flow when my sample or running...

16 February 2021 4,224 3 View

How can I set up UV irradiation for crosslinking lab?

Hi, I am using a mix of 2 polymers and want to try and crosslink them. I have a UVA lamp of 365nm and have tried the lab previously at 30cm above. How would you recommend I approach the lab and...

31 January 2021 8,711 3 View

Which air pollution/air quality books do you recommend for working with environmental engineering students?

I am exploring up-to-date books on air pollution and air quality, and I would like to know which books do you recommend for environmental engineering students.

25 January 2021 3,972 7 View

How to simulate Sloshing water using Dynamic mesh in ANSYS Fluent ?

I am trying to model sloshing water in a tank in ANSYS Fluent using Wall motion. BUT it seems that System coupling is not responding to wall motion and it works only with Dynamic mesh.

20 January 2021 2,963 3 View

Are there other models that meet the same purpose?

Both Jakes and Gaussian models are used to characterize the power spectral densities of mobile communication channels.

11 January 2021 759 3 View

SDAS, secondary dendritic arms space relation with solidification rate?

Want to know the relation between sdas and solidification rate, the mechanism by which getting smaller space between secondary arms by increasing the rate ?

23 December 2020 735 2 View

Shall i use the country's CPI score or rank while entering the data ?

I have the Corruption perception index as a control variable in my study and i'm not sure what i have use the score or the rank of the country ? please advice what i have to use in the data entry...

09 December 2020 3,797 2 View

Any thoughts for a research topic in a master's level thesis regarding combination of Supply chain and entrepreneurship?

Hi, we are two master's students at JIBS and currently looking for inspiration and suggestions for a research topic that has a connection of both "supply chain" and "entrepreneurship". maybe...

18 November 2020 2,524 3 View

Can electrophoretic mobility measurements of polymer-coated AuNPs be used to infer the role of charge (if any) in their dispersion stability?

Hello Everyone, We have stable citrate-coated AuNPs dispersed in water. Different polymeric protective coatings (PEG, PVP, BSA) have been chemically grafted to the citrate-coated AuNPs for...

17 November 2020 2,903 16 View

How can instructors assess the intercultural competence of their ESL students?

Intercultural competence is a skill rarely taught and measured in ESL venues. What is an ideal way of measuring the student's progress in developing intercultrual awareness and testing it?

08 November 2020 2,176 16 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View