At a fixed voltage of 260V, electrophoresis of protein was faster in our previous batch of 1x SDS running buffer. However, the electrophoresis was much slower recently with much lower current (less than half of the previous one). The same issue occurs even with new dilution of freshly prepared 10x buffer to the 1x buffer. What would be the possible reasons of such issue?
It may be too much salt in the buffer. Check that any solvated buffer components are not being weighed as the anhudrous form if previously they were solvated, Also check that nobody has set a low maximim current on the power supply limiting the running sprrd of the samples
Several factors could contribute to a decrease in electrophoresis speed and lower current during protein electrophoresis. Here are some possible reasons for the observed issue:
By systematically investigating these factors, you should be able to identify the root cause of the slower electrophoresis and take appropriate corrective actions.
Check the electrodes. If you see them covered with whitish stuff, remove it with wet tissue or brush until the metal surface is exposed.
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