I'm extracting RNA with a trizol-like reagent called Transzol. It looks like there's variable amounts of gDNA on a high molecular weight band on the agarose gel right above the ribosomal bands.
I'm worried that I'm doing an overquantificaiton of total RNA due to gDNA contamination.
Before starting with the RT, I treat the samples with DNAseI in some 5X First Strand Buffer (1ul, in a total volume of 10ul). But I maybe starting with different amounts of total RNA.
How can I digest the RNA samples with DNAse before RT while not adding buffers that will mess with the RT enzyme?
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