Currently, I'm working on a DNA pol activity test, one of the variables is DNA pol concentration in the PCR/LAMP assay. For LAMP I found a clear protocol for the DNA pol in weights. However, for PCR I found a lot of the protocol in the unit.
How can I compare my DNA pol (grown in the lab and extracted) dose/quantity to the control (Taq master mix) for an apple to apple comparison?
You might start by trying to find out how the unit of Taq polymerase enzyme activity is defined, including all of the conditions used for measuring it. Then you could use that method with your own polymerase preparation to compare the activity of the two preparations.
There are a number of commercial kits that allow you to measure the activity using a real time pcr machine. Google " dna polymerase activity assay". There are also some great papers on the topic that will pop up with this search criteria.
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