I have been using TRIzol/TRIreagent/qiazol to extract both RNA and DNA from liver. I consistently isolate very good RNA. DNA, however, is generally low concentration and has many contaminants.
I have tried smaller/larger sample, longer/shorter homogenization, shaking/vortexing, longer/shorter centrifugation, longer/shorter incubation, dumping/pipetting supernatant, and longer/shorter drying.
It seems as though I have tried everything but I know that there is always something else that can be done. Any hacks would be greatly appreciated.
I have found that putting in an extra chloroform:isoamyl step helps to reduce contamination, also for the precipitation step an extra ethanol step is usually helpful. make sure everything you use for the reverse transcription is scrupulously clean and if necessary do the work in a clean flow hood. I tended to UV tips, gilsons, water etc.
I always do an extra ethanol step when isolating the DNA from Trizol to reduce the contaminants
I think Trizol is mainly manufactured for RNA isolation. Although suppliers claim that TRIzol can give you DNA, RNA and Protein.
We tried this, and found protein isolation as well and did SDS PAGE.
However, I think trizol is best for RNA.
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