Hi all,

I am trying to extract DNA from Alternaria spores. They prove to be very tough to open. Does anyone have a lab protocol they are using to obtain a high yield of Alternaria spore DNA? Currently, I am using the E.Z.N.A. Universal Pathogen Kit and bead milling with a range of bead sizes from 0.1-0.5 mm. I am thinking of trying a pre-DNA extraction step of incubating with lyticase overnight at 30C, or trying a freeze thaw step with LN2.

Any help would be much appreciated!

Cheers,

Melissa

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