Hi, I'm using a very hydrophobic peptide for tetramer staining. The peptide can only be dissolved in DMSO, and then further diluted by PBS or water. I have tried 10, 5, 3 and 2 mg/mL as the stock concentration, and only when it's dissolved at 2 mg/mL in DMSO, it can be further diluted in PBS or water without any aggregation.

However, for tetramer staining, it's suggested to not exceed the final concentration of 10% DMSO in the peptide exchange reaction for tetramer assembly, but in my case, the 2 mg/mL peptide stock leads to a 16% DMSO in the exchange reaction. Does anyone have any experience to somehow lower the DMSO concentration in the reaction? Like add more monomers or add more PBS to the reaction to increase the final volume?

Thanks!