hi
any expert available for determination of dissociation constant by 1H-NMR ?
I want a model that is balanced with accuracy or speed, faster rcnn has high accuracy while yolo have fast speed. i am thinking to combine them to get a hybrid model to achieve both speed and accuracy
02 August 2024 3,104 0 View
Suggest one of them 1. Teflon-lined stainless steel autoclave: 2. Alumina (Al2O3) ceramic container
30 July 2024 7,326 1 View
I've attempted to use this method to synthesize copper nanoparticles. Copper nanoparticles can be synthesised using a variety of precursor materials. CuSO4, distilled water, NaOH, and EDTA are...
28 July 2024 8,027 3 View
Hi everyone, I have extracted DNA from a biogas bioreactor using Qiagen kit and prep cDNA library then used this library as template to optimize primers for qPCR (taken from papers). Some of the...
23 July 2024 1,329 5 View
Hello!!! I want to implement the Swerling characteristics functions (CF) directly in MATLAB without using its Fourier integral pairs...the Swerling CFs are actually Laplace Transform of the signal...
23 July 2024 4,925 1 View
Currently I need to calculate detection probabilities (PD) from RCS data. Beta distribution parameters for this RCS data are calculated and will be used in Swerling0 Equation. The idea is based on...
22 July 2024 2,851 0 View
Why methanol and sulphuric acid, used in the analysis of polyhydroxyalkanoates (PHA) methyl esters by GC-MS? Additionally, why do we typically use non-polar solvents in GC-MS?
22 July 2024 1,210 2 View
Currently I need to calculate detection probabilities (PD) from radar cross section (RCS) data. Beta distribution parameters for this RCS data are calculated and will be used in Swerling0...
22 July 2024 868 0 View
Thanks
22 July 2024 1,143 3 View
Hello I want to know about the sputtering condition of depositing FTO from target 95:5%. I tried with RF sputter in pressure 2.5Pa and 100 sccm Ar at room temperature but it showing no...
21 July 2024 1,680 2 View
I'm facing issues with the tumor dissociation protocol for GBM. I use collagenase/dispase with DNase to dissociate my tumor sample. However, even after incubating it for 60mins, I do not get a...
22 July 2024 412 3 View
I synthesized some paramagnetic cobalt and nickel complexes (expected to be Co(II) and Ni(II)) using a bidentate ligand - N of pyridine ring (aromatic) and O of P=O (aliphatic), and I obtained...
03 July 2024 3,410 4 View
Hello, I got reviver comment " please look into the comparative assessments regarding Ki (inhibition constant); Kd (dissociation constant), and Km "equilibrium constant" of the target...
09 June 2024 10,069 3 View
We produced scFv of an antibody from bacteria. Now we want to establish dissociation Kd of antibody using quantitative elisa. How can i know the expected Kd value (range in nM, or mM)?
04 June 2024 2,602 5 View
I am following these steps to calculate the dielectric constant of a zeolite. https://www.vasp.at/wiki/index.php/Dielectric_properties_of_SiC However, In step 2 IPA (independent particle...
02 June 2024 7,390 0 View
During electrocatalytic nitrogen reduction to ammonia, we perform isotopic labelling test using HNMR to confirm source of ammonia.
29 May 2024 5,165 0 View
At the risk of asking a stupid question. I'm planning to start isotropic fractionator experiments on insects. The protocol says to make a dissociation solution of 1L of 1% triton x-100 in 40mM...
27 May 2024 821 2 View
I perform al alkylation reaction on an imdazole compound where the alkyl reagent (1-Iodo-Octane) was in excess. After the reaction was completed, I then performed an extraction, followed by...
24 May 2024 8,042 2 View
In dielectric, how do we know whether conduction wrt applied frequency takes place due to electron or due to a polaron. In Jonscher power law, can we distinguish whether the conduction is due to...
16 May 2024 4,102 1 View
When I calculated Kd, I found that although the concentrations were set to satisfy the type of binding used to determine the differential spectrum, Kd was unstable if the two high concentrations...
14 May 2024 399 0 View