Direct SDS (Laemmli) Lysate for Western blotting?

08 August 2019 0 7K Report

Dear all, In the past I use a siimple and fast method to prepare my tissue or cell lysates. Usually snap freeze the tissue and after direct dissociation (sonication/ultrathurax) and boiling, protein determination and then WB.

Is there people that use directly Laemmli buffer, sonicate and heat denature before perfoming SDS gel and western blot?

Protocols are welcome!

Marc

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Science method

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