How to remove colour from DNA pellet during DNA extraction?

I am working with Amaranth accessions which are green to red in color and contain high phenolic compounds. Using the CTAB method with the slight modification I am trying to get high-quality DNA so...

18 January 2021 3,578 1 View

How to calculate in-plane lattice strain for 2D material?

I am working with h-BN and need to find out the strain due to in-plane lattice constant. The famous Williamson-Hall method may not be applicable here as it analyzes XRD for calculation of strain...

19 October 2020 7,386 4 View

How to add texture in ggplot?

Hi all, I am using the following code to obtain the bar plot uisng the R package Ggpubr. I am required to add texture into the bar plots instead of color. I could not find any option for adding...

29 July 2020 829 3 View

Response Surface modeling with insignificant p values?

I ran the response surface modeling using central composite design. And I got this output. Can I take this model? The model is having lack of fit p value more than 0.05. But other parameters are...

23 February 2020 6,776 4 View

What physical process determines the property of photon entanglement in a SPE?

For instance, both nitride NW-QDs and h-BN point defect based SPEs seem to exhibit photon entanglement. Which platform will provide stronger entanglement? I suspect, that purity of the single...

19 January 2020 6,442 2 View

Can you Please suggest me journals that publish works on Blockchain and relevant systems free of charge and are scopus indexed?

I am a student and currently have no access to funds. I have extend my previous work ( book chapter) and interested in submitting to a journal. Can anyone please suggest to me some journals that...

25 December 2019 2,239 4 View

Why mice not rats are used for testing efficacy of vaccine?

What is the reason that most of the researcher uses mice for evaluating immune response of vaccines. Why nobody uses rat for the same purpose?

17 November 2019 1,203 3 View

Is highly oriented pyrolytic graphite (HOPG) a good absorber at telecom wavelength (1550 nm)? If yes then what is the remedy for zero absorbtion?

HOPG is a good substrate for growth of h-BN. One major application of h-BN is single photon emission which can be used for telecom wavelength. Can anyone give me any insight about the absorption...

11 September 2019 6,306 1 View

Can I write a research paper if I get negative results ?

I am writing a paper on NLP related problem. I used deep learning, and this was never used before for this specific problem. Apparently the accuracy decreases if the number of data increases. I...

28 July 2019 3,870 34 View

Can we develop PDX model in thymectomized C57bl/6 or Balb/c mice?

Since humanized and SCID mice are very expensive to procure and maintain. I was wondering to develop a cost and effective approach to develop an in vivo model for cancer research. Thymectomy is...

10 July 2019 8,111 4 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Can anyone advise me about a cortisol antibody made in rabbit to set up an ELISA?

I am trying to set up a cortisol EIA using mouse anti-rabbit IGg as coating and a rabbit cortisol antibody as secondary ab. I am searching for a sensitive and specific cortisol Ab made in rabbit

20 February 2021 3,366 5 View