During an ELISA test for plant virus, if the quantity of antiserum and conjugate is less, is it possible to dilute the antiserum and conjugate with water (ultrapure/milliq)?
Dilute the antiserum with a buffer that is used for the conjugate or a suitable buffer for the other materials in the assay.
Dilution must be done with buffered saline.
I agree with Sourabh and Jack. However, I would go one step ahead by saying that do use buffer (not buffered saline) but in pretty low concentration.
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