Dilution of antiserum and conjugate in ELISA?

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Jack Haiden Britt

Dilute the antiserum with a buffer that is used for the conjugate or a suitable buffer for the other materials in the assay.

Jai Ghosh

I agree with Sourabh and Jack. However, I would go one step ahead by saying that do use buffer (not buffered saline) but in pretty low concentration.