Hi
I am working with Mycobacterium tuberculosis variant bovis BCG strain TMC 1011 which requires 12/16 days to grow as visible colonies. I had inoculated my culture on 7H10 slant but before my organism grows the slants and the plate cracks or dehydrates. I have made slants having small surface area and larger butt to prevent dehydration. I have also tried sealing both my slants and plate with parafilm. However with prolonged incubation at 37 C the parafilm turns sticky and it becomes difficult to open plates and plug off slants.
Please suggest some ideas to avoid dehydration of my media.
Regards,
Arti Sharma.
Hi Arti, sounds like your incubator has low humidity. If it doesn't have a humidity control you could add a pan of water to bring up the humidity. Additionally/alternatively, you could seal the plate or slants in a plastic bag. If you are growing on 20mL or more 7H10 you should be able to incubate for at least 4 weeks without significant dehydration.
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