Dear Madam/Sir? Can you clarify my doubt? When I raised the polyclonal antibody in Rabbit against a cucurbitaceous host virus and was evaluating it against the infected leaf samples of N.benthamiana , there was difference in the O.D values between healthy and Infected N.benthamiana, but it is not 2 fold or more than that to consider it as a positive. Generally we take a 2 fold difference between a healthy and infected samples to consider as positive. At that point of time, I was not having infected cucurbitaceous hosts to validate the antibody. Later, when i had the infected samples of cucurbitaceous hosts such as muskmelon, bottlegourd, cucumber and validated against those there was even upto five fold difference between the infected and healthy samples. Is it because the virus multiplies luxuriantly in a cucurbitaceous hosts compared to model plant N.benthamiana which belongs to solanaceae . Or is it because of anyother factors that is responsible for lower reading in N.benthamiana. Can you furnish your ideas or opinion?
Dear Sesha Kiran Kollipara
Several factors affecting hostplant virus multiplication, such as light, Temperature, nutrition, time of the year, plant spp. and var. , incubation condition of inoculated plants…, also part of plant you tested. Pls. find the attached files, Hoping this will be helpful
R C Matthews. 2012. Fundamentals of Plant Virology Academic Press, Pp. 403. ISBN0323138497, 9780323138499
http://onlinelibrary.wiley.com/doi/10.1111/j.1744-7348.1950.tb01040.x/abstract
http://scholar.google.com/scholar_url?url=http://revistes.iec.cat/index.php/IM/article/download/9430/9427&hl=ar&sa=X&scisig=AAGBfm3avn8zTcvE8ONXB1zgLzB1uuEkkQ&nossl=1&oi=scholarr
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2908549/
Regards
Prof. Houda Kawas
Dear Houda Kawas,
Plants were grown in glass house under same conditions. Optimal time of 4 weeks after sap inoculation before taking leaf samples from N.benthamiana and also different cucurbitaceous hosts. Will reconfirm by repeating the sap inoculatiion. Thanks for your suggestion and comments
You most welcome
We sometimes call hostplant for multiplication, hostplant for diagnose symptoms, also same plant spp. and var. not the same reaction to virus isolates under same condition, good luck in your research
Regards
Houda
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