Hi there,
Im working with Hek-293 Cells and when preparing them for an experiment I'm used to seed the cells in a 12-well plate and also seed a stock in a 100mm dish.
When I seed the cells in 12-well plates the cells grow stacked in clusters while in 100-well plates the cells grow with their classic epithelial morphology. I do not use lysine or laminin or any other treatment for 100 or 12-well plates.
My question is why does this happen and if there's any way to avoid it. Can i trust in the results obtained from cells growing with a different morphology?
Thank you in advance
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