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Questions related from Alejandro Febles
Hello everyone. We are recently starting to perform reversible biotinylation experiments. I created a protocol customized to our needs based on different sources (see below). In this protocol, we...
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Hello everyone. I'm reading articles to perform for the first time an internalization assay of membrane receptors with Sulfo-NHS-Biotin. I understand that for cleavage biotin it is necessary that...
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Hi there, Im working with Hek-293 Cells and when preparing them for an experiment I'm used to seed the cells in a 12-well plate and also seed a stock in a 100mm dish. When I seed the cells in...
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In my lab we have the Trans-blot Turbo by BioRad, and I wonder Pros and Cons of this technique against the conventional transfer. I guess the main pro is a quick transfer (~30') but i wanna know...
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