Hi,
Can anyone please highlight the difference between Coomassie Brilliant Blue R-250 and G-250?? Which one of them will stain the SDS gel faster?
Hi, Anyone has first hand experience of publishing in F1000Research. I guess it follows open peer review and does not encourage measurable parameters like impact factor! What are pros and cons...
05 June 2019 9,547 1 View
Hi, I have two proteins that are his tagged at N-terminus. One of them forms hexamer around 370kDa and other is 17kDa. I want to check their interaction and get Kd value. Which of the following...
03 April 2019 1,474 5 View
Hi all, I have a protein with conserved Threonine and one Lysine in the middle of the protein sequence. Being a charged and conserved amino acid across family domain of this protein, I have strong...
02 March 2019 4,847 5 View
Hi, I have cloned some three different constructs which are putative membrane protein of Mycobacterium tuberculosis into pET28a with N-term His tag. I tried to express them in BL21, Rosetta, C41,...
11 December 2018 1,871 3 View
Hi, Can anyone please tell some bioinformatics server that helps in predicting the location of flexible region in a protein.?
10 November 2018 6,510 0 View
Hi, I was just curious to know whether the scientific articles and review published in preprint repository like bioRxiv are citable? If yes, is it good practice to do that since mostly such...
09 October 2018 4,686 4 View
Hi, I want to add about 5 CMC DDM detergent in my Size exclusion buffer. Is it advisable to add detergent and then degass by vacuum or degass the buffer first and then add DDM dropwise slowly??
08 September 2018 4,062 3 View
Hi, One of my colleague put the Superdex 200 10/300 GL column mistakenly in 1M NaOH at flowrate of 0.2ml/min overnight instead of water wash. Will the column be stable? How to know that the...
07 August 2018 5,171 1 View
Hi, Can someone please tell pros and cons briefly of publishing in preprint servers like bioRxiv?
07 August 2018 7,602 1 View
Hi, I injected my protein in Sephadex 200 column with a flow rate of 0.2 ml/min. The UV baseline is not stable and I am afraid what is happening? Can anyone guide me what is happening? See...
07 August 2018 1,576 0 View
Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...
03 March 2021 8,237 3 View
I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...
02 March 2021 6,270 3 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...
28 February 2021 7,370 3 View
I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...
27 February 2021 9,176 3 View
I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...
25 February 2021 8,140 3 View
Greetings, I want to make SDS PAGE for my sample (Tick salivery gland extract), I tried concentrations 10%, 12%. I also tried high molecular weight ladder. But the protein stopped at the top and...
25 February 2021 7,698 3 View
I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...
24 February 2021 8,325 1 View
I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...
24 February 2021 6,310 3 View
Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...
24 February 2021 5,749 3 View