Hi,
Can anyone please tell some bioinformatics server that helps in predicting the location of flexible region in a protein.?
Hi, Anyone has first hand experience of publishing in F1000Research. I guess it follows open peer review and does not encourage measurable parameters like impact factor! What are pros and cons...
05 June 2019 9,763 1 View
Hi, I have two proteins that are his tagged at N-terminus. One of them forms hexamer around 370kDa and other is 17kDa. I want to check their interaction and get Kd value. Which of the following...
03 April 2019 1,604 5 View
Hi all, I have a protein with conserved Threonine and one Lysine in the middle of the protein sequence. Being a charged and conserved amino acid across family domain of this protein, I have strong...
02 March 2019 4,959 5 View
Hi, I have cloned some three different constructs which are putative membrane protein of Mycobacterium tuberculosis into pET28a with N-term His tag. I tried to express them in BL21, Rosetta, C41,...
11 December 2018 1,979 3 View
Hi, Can anyone please highlight the difference between Coomassie Brilliant Blue R-250 and G-250?? Which one of them will stain the SDS gel faster?
09 October 2018 3,062 0 View
Hi, I was just curious to know whether the scientific articles and review published in preprint repository like bioRxiv are citable? If yes, is it good practice to do that since mostly such...
09 October 2018 4,812 4 View
Hi, I want to add about 5 CMC DDM detergent in my Size exclusion buffer. Is it advisable to add detergent and then degass by vacuum or degass the buffer first and then add DDM dropwise slowly??
08 September 2018 4,241 3 View
Hi, Can someone please tell pros and cons briefly of publishing in preprint servers like bioRxiv?
07 August 2018 7,726 1 View
Hi, One of my colleague put the Superdex 200 10/300 GL column mistakenly in 1M NaOH at flowrate of 0.2ml/min overnight instead of water wash. Will the column be stable? How to know that the...
07 August 2018 5,344 1 View
Hi, I injected my protein in Sephadex 200 column with a flow rate of 0.2 ml/min. The UV baseline is not stable and I am afraid what is happening? Can anyone guide me what is happening? See...
07 August 2018 1,745 0 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am looking for software to help analyze SELEX NGS data, including alignment, sequence enrichment, and other related tasks. Can anyone recommend suitable tools or software? Best wishes, Waleed
30 July 2024 1,061 5 View
PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...
28 July 2024 4,762 2 View
Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...
28 July 2024 6,938 1 View
I am reaching out to seek your valuable advice and recommendations regarding the best software tools to use for this research. Specifically, I am looking for software with a user-friendly...
22 July 2024 3,794 1 View
What type of experimental design do l use and what statistical tools do l use for the hypothesis. What is the position of control group in the analysis?. Thanks
18 July 2024 3,581 3 View
To help enchancing patients flow and service efficiency
18 July 2024 482 2 View
I am a 3rd year Computer Science student currently writing our Bachelor's thesis about finding diverse k-shortest paths in pedestrian networks. We have chosen 3 local areas as our proposed...
15 July 2024 4,289 0 View
Hi all, Do you know how to plot this figure? I found many of them in Nature journals but have not figured out how to make them. Which software/tool do you suggest? Thank you very much.
15 July 2024 8,384 2 View
Bioinformatics tools like peptide cutter
15 July 2024 6,453 1 View