Hi,
I injected my protein in Sephadex 200 column with a flow rate of 0.2 ml/min. The UV baseline is not stable and I am afraid what is happening? Can anyone guide me what is happening?
See attached photo.
Hi, Anyone has first hand experience of publishing in F1000Research. I guess it follows open peer review and does not encourage measurable parameters like impact factor! What are pros and cons...
05 June 2019 9,763 1 View
Hi, I have two proteins that are his tagged at N-terminus. One of them forms hexamer around 370kDa and other is 17kDa. I want to check their interaction and get Kd value. Which of the following...
03 April 2019 1,604 5 View
Hi all, I have a protein with conserved Threonine and one Lysine in the middle of the protein sequence. Being a charged and conserved amino acid across family domain of this protein, I have strong...
02 March 2019 4,959 5 View
Hi, I have cloned some three different constructs which are putative membrane protein of Mycobacterium tuberculosis into pET28a with N-term His tag. I tried to express them in BL21, Rosetta, C41,...
11 December 2018 1,979 3 View
Hi, Can anyone please tell some bioinformatics server that helps in predicting the location of flexible region in a protein.?
10 November 2018 6,649 0 View
Hi, Can anyone please highlight the difference between Coomassie Brilliant Blue R-250 and G-250?? Which one of them will stain the SDS gel faster?
09 October 2018 3,062 0 View
Hi, I was just curious to know whether the scientific articles and review published in preprint repository like bioRxiv are citable? If yes, is it good practice to do that since mostly such...
09 October 2018 4,812 4 View
Hi, I want to add about 5 CMC DDM detergent in my Size exclusion buffer. Is it advisable to add detergent and then degass by vacuum or degass the buffer first and then add DDM dropwise slowly??
08 September 2018 4,241 3 View
Hi, Can someone please tell pros and cons briefly of publishing in preprint servers like bioRxiv?
07 August 2018 7,726 1 View
Hi, One of my colleague put the Superdex 200 10/300 GL column mistakenly in 1M NaOH at flowrate of 0.2ml/min overnight instead of water wash. Will the column be stable? How to know that the...
07 August 2018 5,344 1 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
A website software of Blackbody radiation law expert software can used through the following web site. http://39.105.188.151:3000/index
07 August 2024 1,706 0 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I am trying to recover liquid CO2 from a mixture of 0.6 CO2, 0.3 N2, and 0.1 O2. My aim is to recover about 99% liquid CO2 at the bottom of the column and make sure the amount of gaseous CO2...
06 August 2024 4,611 0 View
How can I select cells in prime editing without fluorescence-activated cell separation?
31 July 2024 8,551 2 View
I'm researching light sources and their UV emissions. I'm curious if a 50W, 7500K conventional LED lamp emits any UV light. I found that a 200W tungsten lamp can emit up to 1.5 mW/cm² at 350 nm....
31 July 2024 3,843 7 View
Hello Everyone I have a question about structure for connectivity analysis on sources. My goal: preprocess and cut data into trials create headmodels, using template MRI file perform source...
30 July 2024 2,744 1 View