Can anyone advise me about a cortisol antibody made in rabbit to set up an ELISA?

I am trying to set up a cortisol EIA using mouse anti-rabbit IGg as coating and a rabbit cortisol antibody as secondary ab. I am searching for a sensitive and specific cortisol Ab made in rabbit

20 February 2021 3,366 5 View

How to select from various invitations from Scientific Boards in conferences and proceedings?

How to select from various invitations from Scientific Boards in conferences and proceedings? How to spot the ones with high impact and genuine? Can we spot valuable events?

02 February 2021 8,187 1 View

Can R be used for SEO?

13 January 2021 1,920 1 View

Fish gut microbiota extraction and identification protocol?

Hi, I need a protocol to extract and identify microbiota from fish gut. Any suggestions? Thank you. (P.s. Something low tech as I am technologically limited, guess no funds and big high tech...

01 January 2021 808 1 View

Bisphenol A in water - measuring bpa protocol using a uv/vis spectrophotometer?

Hi, I'm interested in a protocol for measuring concentration of BPA in distilled water (no interference) using a uv/vis spectrophotometer . The method found...

09 November 2020 7,012 3 View

How to remove ethanol from a 95% water solution?

How can ethanol be removed from an azeotropic 95% water 5% ethanol solution, without reaching more than 30 C temp?

06 November 2020 8,718 2 View

Bisphenol A solubility in water protocol?

Upon some research I found different protocols of BPA solubility, some state a 5 v/v % ethanol (MOTOYAMA et al ) will catalyze the process of solubility and BPA will stay in solution (being an...

06 November 2020 8,152 8 View

Proceedings Of International Conference Building Services And Energy Efficiency is Scopus indexed?

Proceedings Of International Conference Building Services And Energy Efficiency is Scopus indexed? see link here https://content.sciendo.com/view/title/580364

05 November 2020 4,157 3 View

Probable Identity table of halophilic bacteria?

I need older protocols that predate pcr, on halophilic or halotolerant bacteria. Looking forward to receiving your answers, thank you.

13 October 2020 8,022 5 View

Is there a valid alternative way to replace the use of camera lucida (or drawing tube) for scientific and taxonomic drawings?

So far I have not been able to find a camera lucida that fits a low range microscope.

05 September 2020 3,342 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

How to show in a scientific paper format the post-hoc test of 4 variables involved, when 3 by 3 variables are significant?

In a study involving 4 variables (species(2x), temperature(2x), nutrients(2x) and light(3x)), 3 by 3 variables, for example, "species", "temperature" and "nutrients" are significant. I end up with...

15 February 2021 8,018 2 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View