There is no ideal method. Personally, I recommend: Arrigoni, E., A. Caprez, R. Amado and H. Neukom Z. Lebensm. Unters. Forsch. 178, 195 (1984); and chromatographic identification of soluble polysaccharides
ADF and NDF are no dietary fiber. Here is applicable method of Van Soest
Commonly methods are:
1) Prosky Method (AOAC 985.29)
2) McCleary Method (AOAC 2009.01)
The last one measures all components of dietary fiber as currently defined by CODEX Alimentarius. But very often there is a risk to underestimate or overestimate some components with these methods.
It seems you are going to measure total dietary fibre. If so the best method is to use the kit that meaure almost 100 sample. Please go to Sigma and you will find it easily.
There are methodS just not one single method. The AOAC has done a great job on this, with methods now also for oligosaccharides (but there are not the enzymatic-gravimetric method). Measuring reliably dietary fiber has been a topic since the 1980's I guess as the definition is functional not chemical.
The AOAC methods are the "officials" but the modifications are capables, and the method you will use, depends of your samples.
It's a matter of what you want from the DF measurement: if you want to measure DF then at one point or another you must use the official methods and confront whichever method you have chosen to that. The officials methods in particular are adapted to cereals rather than fruit & veg for example. You really need to know and understand what you are doing. Otherwise f you want to study composition, then I'd go for a cell wall preparation method, not a DF method for example.
Hi Gabriele, you can separate soluble and insoluble DF and use gas chromatograph to analyse the content of each sugar present in each fraction through the alditol acetates technique .
I've attached a paper where they use this protocol.
Thanks for your answer, I will use the "Integrated Total Dietary Fiber AOAC 2009.1/2011.25 kit" by Megazyme to correctly analyze all components of dietary fiber.
Dear Gabriele,
What you're going to use for your analysis is quite reliable. And you can employ various other methods to analyse components such as crude fibre, ADF, NDF and so on. I think if you can employ two different conventional methods to anlyse DF and compare the results much better.
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